FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264501
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG1750.001508434377.196915240-244
CCGAGTA23550.068.381062
CGAGTAG23400.066.625953
AGTAGCT80900.062.968745
ATCGTAA3550.00376036461.30687245
GAGTAGC51450.060.8537674
GTAGCTG84850.059.7349176
CGATCGT1301.8768909E-455.804974245
AAGTGCT93550.054.3854145
TAGCTGG97200.054.2573477
GCTGGGA190250.054.1585779
CTACTCG23400.054.0136342
TCGCGTA800.00507783952.77736230-234
CGTGCAA6651.7407729E-952.491642235-239
TACTCGG23800.052.2971233
CCCGAGT33050.050.917691
CCAAAGT109800.050.8358352
AAAGTGC101600.050.7078134
CAAAGTG110350.048.7219543
CCCAAAG126050.048.7140161
Produced by FastQC (version 0.11.5)