FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4649961
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16950.044.2389073
CCGAGTA18050.043.6331182
TACTCGG14500.040.655473
CTACTCG14250.040.0449262
GCTGGGA144550.039.355429
CCCAAAG95650.039.1066471
AAAGTGC80300.039.055584
AAGTGCT86950.037.0448765
CCAAAGT92400.036.748322
AGTAGCT72550.036.40225
GAGTAGC46100.036.2142334
GCTACTC35150.036.0097271
GTAGCTG73900.035.9806636
CCCGAGT22850.035.964451
TAGCTGG79900.035.0999157
CAAAGTG92550.035.0071953
AGTGCTG91350.034.09596
GTGCTGG93100.032.9489827
GGAGGCT105300.031.6297959
TTGGGAG95300.031.0066576
Produced by FastQC (version 0.11.5)