FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466865
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT5250.002886196665.53407340-345
AGTAGCT96600.063.4829525
GCGAATA2451.3341874E-463.009342340-345
GTAGCTG100850.060.9460036
CGAGTAG28200.059.913243
GAGTAGC67150.059.5370754
CCGAGTA28900.059.4243932
GCTGGGA205300.056.2592479
TAGCTGG112650.055.9143457
AGTCCGT6300.005941257354.61173340-345
AAGTGCT101500.053.4996155
AAAGTGC111200.048.395154
CCCGAGT36350.048.23581
CCAAAGT123200.046.8417432
CCCAAAG135050.046.6259161
AGTGCTG114050.045.7414476
TACTCGG23850.045.4780543
TGAGTAG50150.045.3359223
CAAAGTG122450.045.0277333
CTACTCG24800.044.296672
Produced by FastQC (version 0.11.5)