FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466865
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA26900.065.2105262
CGAGTAG27450.063.1938973
AGTAGCT90550.062.8514945
GTAGCTG94250.061.1423656
GAGTAGC62250.061.0552144
ACGATTA3650.00467079558.04179245
CCCGAGT32350.054.6798021
TAGCTGG106100.054.4972467
GCTGGGA204600.054.1083039
AAGTGCT99450.053.176485
CTAGACG5503.6615922E-451.358192245
TACTCGG26700.050.369233
TTGGGAG129800.050.3527576
CTACTCG26600.050.0701032
AAAGTGC107550.049.47434
CCAAAGT119050.048.3515622
AGTGCTG109550.048.1552476
CAAAGTG116300.047.3162083
GGAGGCT145550.046.4663439
GCTACTC59000.046.404751
Produced by FastQC (version 0.11.5)