FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3729342
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG2404.0674422E-879.97428200-204
CGCAATT2750.001923537972.60649245
AGTAGCT105350.067.028975
CGAGTAG28250.065.228443
GTAGCTG108300.064.899336
GAGTAGC67550.062.7419324
CCGAGTA30050.062.416262
TAGCTGG124350.058.74557
GCTGGGA236550.055.9196789
CTACTCG29750.055.745652
TACTCGG31500.055.3646743
AAGTGCT112450.054.3692865
TTGGGAG146550.054.112636
CAAGTAG46800.053.295533
CCCGAGT37000.051.4677731
CCAAGTA52300.050.9624982
AGTGCTG122900.050.1211856
GGAGGCT173750.049.655749
AAAGTGC122500.049.640174
GCTACTC67450.049.2365721
Produced by FastQC (version 0.11.5)