FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053531
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32950.066.150163
CCGAGTA33700.066.0840152
AGTAGCT110300.063.5791475
GTAGCTG111750.061.6409656
GAGTAGC74100.059.6520584
GCTGGGA232250.055.5960049
AATTGCG3502.5160474E-455.42039310-319
TAGCTGG127250.055.246457
AAGTGCT117100.053.3550155
CCCGAGT42150.052.7215461
TTGCGCG2300.00819835150.35325330-339
TTGGGAG136650.050.1618086
CTACTCG26800.049.5053022
TACTCGG27850.048.611083
AAAGTGC131150.046.8649334
CAAGTAG49100.046.735693
AGTGCTG130200.046.3706636
GGAGGCT150700.045.9775169
CCAAAGT145650.045.7314572
CCCAAAG158250.045.509431
Produced by FastQC (version 0.11.5)