FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053531
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG30000.069.0711443
AGTAGCT101600.065.50185
CCGAGTA31400.065.384252
GTAGCTG105800.062.3007626
GAGTAGC68650.061.0161934
ACGAGTG6408.3437135E-658.36161245
GCTGGGA227300.057.9416169
AAGTGCT116850.056.681235
TAGCTGG121800.056.5173037
CCCGAGT38850.054.5466421
TTGGGAG148550.052.2448276
AAAGTGC128400.052.176614
ACGCATA2650.00714865252.127502235-239
CCAAAGT141750.050.93962
AGTGCTG127750.050.899676
CTACTCG31800.050.5700842
TACTCGG31950.050.133723
CAAAGTG141150.049.580023
CCCAAAG159550.049.4184721
GGAGGCT165000.049.0778779
Produced by FastQC (version 0.11.5)