FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3812896
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT106350.070.347195
CGAGTAG31150.068.2938843
CCGAGTA31750.067.822912
GTAGCTG109700.067.07136
GAGTAGC72450.066.0893554
TAGCTGG124400.060.4007457
CTACTCG30500.059.2975962
GCTGGGA232400.059.092569
TACTCGG30950.057.8048323
CCCGAGT39100.057.303481
TTGGGAG149350.055.3631826
AAGTGCT120550.054.2357035
GCTACTC67200.053.3080331
CAAGTAG46450.052.521573
GGAGGCT169350.051.9364439
GCACTTT123700.050.8108981
CCAAGTA49150.050.695262
AATAGCG4050.00805876650.567505245
CTCGGGA36900.050.42275
AGTGCTG126250.050.395086
Produced by FastQC (version 0.11.5)