FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155698
Sequences flagged as poor quality0
Sequence length35-351
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT106900.078.275325
GTAGCTG109100.075.9896246
CGAGTAG33300.073.9186253
CCGAGTA34800.073.000872
GAGTAGC76600.071.107764
TAGCTGG125300.068.730687
GCTGGGA227750.065.096539
AAGTGCT105300.062.699415
CCCGAGT42050.061.6641
CAAGTAG42500.059.9436843
AAGTAGC46050.056.8810044
TTGGGAG137550.056.524896
TGAGTAG51250.055.3104553
CGCTATA3605.7863268E-555.032894340-345
CCAAGTA47250.054.9807622
AGTGCTG117800.054.940636
CTACTCG24950.054.074352
AAAGTGC122850.053.9760134
CCAAAGT133900.053.755682
CTGAGTA53250.053.50262
Produced by FastQC (version 0.11.5)