FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155698
Sequences flagged as poor quality0
Sequence length35-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28700.074.5972063
AGTAGCT94250.074.05295
GAGTAGC64100.071.5566564
GTAGCTG97550.070.732426
CCGAGTA29950.070.598562
TCGAATG5601.2307282E-768.14632245
AAGTGCT97300.064.170165
TAGCTGG112000.062.493397
GCTGGGA210600.062.4412359
TTGGGAG145750.058.937476
CCCGAGT36650.058.1259541
CTACTCG30450.056.3786162
TTTGGGA124250.055.4260865
AGTGCTG113500.054.9526756
AAAGTGC114950.054.8369754
GCACTTT113900.054.6535261
TACTCGG31050.054.648763
GGAGGCT156700.054.3105559
CCAAAGT122500.054.0544362
CAAGTAG38400.053.8549163
Produced by FastQC (version 0.11.5)