FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2861196
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10900.048.4451033
CCGAGTA11750.046.545582
GAGTAGC25900.042.9606254
CCCGAGT13450.042.125241
CCCAAAG60900.041.4006771
GCTGGGA86250.041.337519
AAAGTGC50550.040.29224
AGTAGCT40750.040.1476555
TACTCGG8750.039.8732833
AAGTGCT52700.039.0062525
CCAAAGT57400.038.3586542
GTAGCTG42100.037.607136
GCTACTC20750.037.5447581
AGTGCTG56350.037.1846166
CTACTCG9650.037.1316832
TAGCTGG44700.036.991067
CAAAGTG58250.036.2610553
GTGCTGG58150.035.059177
CGGAGGT13600.033.1584327
TGCTGGG64550.032.8930248
Produced by FastQC (version 0.11.5)