FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161031
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23400.069.877233
CCGAGTA24450.067.447962
AGTAGCT81550.067.178115
GTAGCTG86400.062.338486
GAGTAGC59000.062.060464
TAGCTGG95500.059.0230837
GCTGGGA188600.058.296449
CCCGAGT29600.056.234131
AAGTGCT98050.055.4456755
CTACTCG21050.051.1215822
CCAAAGT114100.050.7084542
AAAGTGC106700.050.5578464
AGTGCTG107050.050.1831786
CCCAAAG127200.049.2096981
CAAAGTG114000.049.1592453
TACTCGG21950.048.7071343
GTGCTGG114750.046.8142857
GCTACTC47300.046.7240751
TTGGGAG109150.044.4299626
AAGTAGC38100.043.4669344
Produced by FastQC (version 0.11.5)