FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161031
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22900.068.630733
CCGAGTA25050.064.555072
AGTAGCT78900.061.8964655
GAGTAGC52300.060.9703254
GTAGCTG83350.058.6697656
GCTGGGA180500.057.09769
AAGTGCT94350.056.9919365
CTACTCG23650.056.843122
TATCCGA1850.00528520456.263565210-214
CCCGAGT30050.055.4098971
GCTACTC53500.054.1000981
TAGCTGG93200.054.0717967
TACTCGG23950.053.9617843
AAAGTGC98900.053.715024
AGTGCTG103550.051.363996
TTGGGAG116950.051.1427886
GGAGGCT130650.050.799529
CCAAAGT108400.050.325572
CCCAAAG123100.049.7719541
CAAAGTG108650.049.014313
Produced by FastQC (version 0.11.5)