FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3531778
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT1800.001167283282.34824250-259
AGTAGCT103550.070.498965
CGAGTAG32000.068.215413
GTAGCTG111300.066.308576
CCGAGTA34550.064.42362
GAGTAGC74200.062.5998734
GCTGGGA233350.061.230789
TAGCTGG125400.060.840637
AAGTGCT112250.058.5314065
GGACGTA3802.3549933E-456.165546260-269
TTGGGAG148250.055.4628376
CCCGAGT43150.053.7765461
AGTGCTG124500.052.4561046
TTTGGGA132300.051.2298165
AAAGTGC127950.051.1815534
CTACTCG26600.050.852562
CCAAAGT139100.050.1146282
CAAGTAG46750.049.601643
CCCAAAG157200.049.1128231
GGAGGCT156150.048.9966439
Produced by FastQC (version 0.11.5)