FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793240
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTC2354.6018674E-4104.06567320-329
CCGAGTA25600.079.1527562
AGTAGCT90850.076.94855
CGAGTAG26050.076.118613
GTAGCTG95900.073.256486
CCGATAA3200.002160019270.50944310-319
GAGTAGC62250.070.101284
GCTGGGA193100.065.920699
AGTCCGT5851.0808327E-465.7283340-345
AAGTGCT91250.065.27025
TAGCTGG111600.064.697517
CCCGAGT34800.059.101551
CAAGTAG39350.057.5635533
CGTATCT4250.004832120657.542194320-329
TTGGGAG116350.056.6573376
AAAGTGC105250.056.244394
AGTGCTG104450.055.923186
CCAAAGT111600.055.897332
CAAAGTG111500.054.714283
TTTCGTA5300.00602806254.411873340-345
Produced by FastQC (version 0.11.5)