FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362433
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT93100.064.981795
CGAGTAG27450.063.5650633
GTAGCTG99600.061.8261266
CCGAGTA28600.061.5091972
AAGTGCT103500.059.9722565
GCTGGGA208500.059.8204279
CGCAGTT7158.883242E-657.743965310-319
TAGCTGG110100.057.6788837
GAGTAGC63750.056.4233364
AGTGCTG114850.054.238026
TTGGGAG123100.053.6751826
AAAGTGC116700.052.4534264
TACTCGG23600.052.117963
CTACTCG23250.050.7495272
CCAAAGT123550.050.471332
GGAGGCT137750.049.675379
GTGCTGG127150.049.5516937
CCCGAGT36550.049.3387871
CAAAGTG123900.049.2898563
CCCAAAG138850.048.4459271
Produced by FastQC (version 0.11.5)