FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362433
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT950.0088083055134.83957245
CTATCGA1854.639307E-4103.86291245
AGTAGCT84350.068.017035
GTAGCTG87400.067.0116
CGAGTAG24000.066.679883
CCGAGTA25800.064.344182
GAGTAGC55450.063.3494834
TCGACGA2000.003842273360.981636240-244
GCTGGGA190950.060.8913279
TAGCTGG101350.059.4254537
AAGTGCT101500.058.617995
CAAGTAG38500.056.572073
TACTCGG28450.056.0167543
CCCGAGT31200.055.394481
CTACTCG28250.055.2380562
TTGGGAG131150.054.83396
AAAGTGC109900.053.8355264
AGTGCTG113450.053.4971586
CCAAAGT120750.051.9677162
CCAAGTA42150.051.8307272
Produced by FastQC (version 0.11.5)