FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175424
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATG3758.042662E-8127.328865340-345
CGAGTAG29350.073.0918053
AGTAGCT100200.071.631535
GTAGCTG103100.069.29186
CCGAGTA32700.067.160512
TAGCTGG114100.064.070597
GAGTAGC70050.062.0794454
GCTGGGA213500.061.7560929
AAGTGCT104650.057.2588655
CCCGAGT39500.056.0155451
TCTAACG2250.006056048454.360195320-329
TTGGGAG130600.052.757576
CAAGTAG45700.052.5112723
AGTGCTG114400.051.289316
AAAGTGC118950.050.4975554
CCAAAGT127500.050.476342
AAGTAGC48800.049.771584
CCCAAAG140550.049.7650681
CCAAGTA50050.049.6908952
GGAGGCT139400.048.48839
Produced by FastQC (version 0.11.5)