FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175424
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG29100.073.293363
AGTAGCT94200.072.409195
CCGAGTA29500.072.072192
GAGTAGC60300.070.184714
GTAGCTG97400.069.41056
CCCGAGT34100.063.2291831
TAGCTGG110200.062.0177767
GCTGGGA207950.060.82849
AAGTGCT98200.059.214935
CTACTCG26750.057.9186822
TTGGGAG138250.056.4691736
TACTCGG28500.056.009613
AGTGCTG109350.052.7476656
CCAAAGT120550.052.5769122
AAAGTGC112050.052.4950334
AAGTAGC42450.052.139484
CCCAAAG136450.052.080971
GCTACTC61600.052.0121271
TTTGGGA119000.051.7391555
CCAAGTA43050.051.4127962
Produced by FastQC (version 0.11.5)