FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871311
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG49700.027.5491141
AAAGTGC43500.025.4789354
CCAAAGT48200.025.3428462
GCTGGGA69400.025.1142229
CGAGTAG8000.024.7606453
AAGTGCT46850.023.4557295
AGTGCTG48300.023.3461256
CAAAGTG50250.023.0888183
CCGAGTA8450.022.8838882
GTGCTGG46300.022.8300487
ACCGCGC4100.021.79056430-31
TGCTGGG51400.021.1132038
GAGTAGC19250.021.0702844
CTACTCG6600.020.7231962
AGTAGCT34300.020.212775
TAGCTGG37750.019.91177
TTGGGAG43000.018.7936296
TACTCGG7400.018.482853
GTAGCTG37100.018.3630496
GGAGGCT46800.018.2696749
Produced by FastQC (version 0.11.5)