FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871311
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8250.031.2902622
TACTCGG8650.030.385923
CGAGTAG7300.026.3609753
GCTGGGA69700.025.4542039
GAGTAGC18700.024.5971574
AAAGTGC44750.024.5427554
GCTACTC21700.024.484931
CCGAGTA8200.024.0400812
CCCAAAG54500.023.5530031
AAGTGCT47750.023.0990945
CCAAAGT52200.022.8382952
TCGGGAG12350.022.802626
GTGCTGG46800.022.6653827
CAAAGTG51450.022.350193
CTCGGGA12050.022.2018035
AGTAGCT35150.022.165795
TTGGGAG47000.021.4704486
ACTCGGG12200.021.15944
AGTGCTG48800.021.0632216
GGAGGCT52850.020.8700439
Produced by FastQC (version 0.11.5)