FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669296
Sequences flagged as poor quality0
Sequence length35-251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5050.068.781713
CTACTCG5250.066.161452
CGAGTAG3600.059.887523
ACTCGGG6650.055.834984
GCTACTC10950.055.785641
GCTGGGA34050.053.8196269
CTCGGGA6900.052.076115
CGGAGGT5750.052.0761077
GTAGCTG15450.051.1660426
TAGCTGG17100.051.132047
TCGGGAG7200.049.9062656
CCGAGTA4400.048.9988822
AGTAGCT15650.048.9814875
GGAGGCT27650.048.949669
GAGTAGC9250.046.6151544
CCCAAAG28500.042.4465941
GGAGGTT15300.042.2735448
CCCGAGT5850.040.9487341
CCAAAGT27900.038.207812
TTGGGAG23050.037.9330986
Produced by FastQC (version 0.11.5)