FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722286
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5500.057.97793
CCGAGTA6400.054.807232
GCTGGGA46850.054.791289
AGTGCTG36550.044.058446
GTGCTGG37450.042.999637
CCCGAGT8250.042.5171241
GAGTAGC12950.041.860494
CCCAAAG41050.041.5590671
TGCTGGG41100.041.5085038
GTAGCTG23050.041.5026136
AAAGTGC35650.040.698374
AGTAGCT23150.040.634625
TAGCTGG24500.040.347887
CGTAAGG1600.001277320139.8598062
CCAAAGT41400.038.8970072
AAGTGCT38550.038.8775255
CAAAGTG41750.036.2795833
CTACTCG4704.5656634E-1033.9232372
ACATCGG2352.054476E-433.9232372
TACTCGG5059.840733E-1031.5721233
Produced by FastQC (version 0.11.5)