FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614842
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG5700.089.996162
TACTCGG6000.088.168123
GCTACTC13450.087.006051
CCGAGTA5250.085.496362
GCTGGGA48050.081.403929
CGAGTAG5150.080.9310153
CCCGAGT6600.075.295091
AGTAGCT21450.073.987235
GGAGGCT32450.073.607229
TCGGGAG7850.073.5159766
TAGCTGG22900.072.8025747
GAGTAGC12900.072.075424
GTAGCTG22300.071.885956
GTGCTGG34150.066.657117
ACTCGGG7700.066.620544
CTCGGGA8450.064.501695
CCAAAGT35950.062.873712
TGCTGGG39050.061.9875958
CCCAAAG39650.061.858181
AGTGCTG37000.061.0894556
Produced by FastQC (version 0.11.5)