FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871537
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA57850.079.116359
CGAGTAG6700.075.901833
CCGAGTA7650.068.55352
GTGCTGG41500.067.780117
TAGCTGG29650.067.534077
GTAGCTG27650.066.671466
GAGTAGC16400.064.9244164
TGCTGGG47150.063.028518
AGTAGCT28650.062.6802865
CTACTCG6250.061.0250742
AGTGCTG45800.060.722516
CCCAAAG54400.060.179071
GGAGGCT40000.058.800219
CCAAAGT50800.058.499892
AAAGTGC46450.057.4778754
CAAAGTG49800.057.4407733
TACTCGG6950.057.165283
AAGTGCT48150.057.0987975
CCCGAGT9300.054.6819761
TCGGGAG8950.053.2690966
Produced by FastQC (version 0.11.5)