FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871537
Sequences flagged as poor quality0
Sequence length35-251
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG4950.086.4597553
CCGAGTA5250.081.5191962
GCTACTC13650.066.1907961
TACTCGG5500.064.844813
GCTGGGA45850.064.821249
CCCGAGT6900.063.7484051
GAGTAGC11300.061.019164
GGAGGCT34550.060.5593649
CTACTCG6100.060.4155242
AGTAGCT19700.060.346275
GTAGCTG20050.057.514066
CCCAAAG40200.054.1181
TAGCTGG22500.053.364887
GTGCTGG34700.051.389987
AAGTGCT34650.050.777955
AAAGTGC34450.050.7276544
TCGGGAG7800.050.29636
TGCTGGG37350.049.971888
AGTGCTG35350.049.4361426
CAAAGTG38250.048.485273
Produced by FastQC (version 0.11.5)