FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812688
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA62150.094.215279
CGAGTAG7100.092.1344153
CCGAGTA8050.079.279422
GTGCTGG46900.076.202917
AGTAGCT27550.075.86585
GTAGCTG27750.075.3190166
TAGCTGG28700.074.4936457
CCCAAAG54950.074.330771
GCTACTC20450.074.11851
GGAGGCT40200.073.821569
AAGTGCT49200.072.964875
CCAAAGT51650.072.592862
AGTGCTG50150.072.537126
AAAGTGC47800.072.431624
GAGTAGC17850.072.4007644
TGCTGGG54350.071.922198
CAAAGTG50200.071.829213
CCCGAGT10150.067.5925451
CTACTCG7650.066.73982
TACTCGG8300.063.4354823
Produced by FastQC (version 0.11.5)