FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620795
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG200.00527843160.03949
TACTCGG4950.084.06113
CGAGTAG3950.076.9809653
CTACTCG5000.076.818912
CCGAGTA4200.076.209232
GCTGGGA42050.072.3126759
GCTACTC12700.065.527941
CTCGGGA6450.062.0307735
GCGGGTT1552.3835855E-761.950739
TCGGGAG7000.061.7294736
TACCGGG800.00409358460.0147745
GGAGGCT32150.059.2369779
ACTCGGG6850.058.4085354
GAGTAGC11250.058.325474
TAGCTGG19000.058.1195647
AGTAGCT18250.056.12345
CCCAAAG38850.055.612141
CCAAAGT35200.054.5588872
GTGCTGG33100.053.6687977
CAAAGTG34950.053.1175083
Produced by FastQC (version 0.11.5)