FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620795
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4250.084.4628452
TACTCGG4700.076.375983
CGAGTAG3450.076.302183
GCTACTC11650.071.8961261
CCGAGTA4050.067.9526142
TCGGGAG5650.067.76966
GGAGGCT26400.065.7199869
GCTGGGA30500.063.9471479
CTCGGGA6000.061.8221175
AGTAGCT14050.059.614945
ACTCGGG6150.058.368634
GAGTAGC7950.057.193924
GTAGCTG14800.054.1684576
TAGCTGG15750.053.940037
GCTACTT11900.051.281011
CTACTTG10600.050.797232
CCCGAGT5350.049.2042121
CCCAAAG29000.049.1000981
CCAAAGT26650.048.0418742
GTGCTGG24100.046.6706857
Produced by FastQC (version 0.11.5)