FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677334
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6650.0110.589693
CTACTCG6700.0105.093572
TCGGGAG8300.098.031066
GCTGGGA54000.090.9859549
CTCGGGA8850.090.170685
AGTAGCT23050.084.855025
CGAGTAG5950.084.153363
ACTCGGG9000.083.452084
TAGCTGG24250.083.2377
GAGTAGC15300.080.793074
GCTACTC16700.080.578731
CCGAGTA7100.079.338252
CCAAAGT43350.078.6875152
AGTGCTG39350.077.9380956
GTGCTGG39300.077.240957
GGAGGCT39550.076.75279
GTAGCTG24700.076.652596
AAAGTGC41000.076.3281254
CCCAAAG50400.075.131711
AAGTGCT42300.074.352255
Produced by FastQC (version 0.11.5)