FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677334
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6050.0102.176772
CCGAGTA4900.094.617782
CGAGTAG4800.091.6357043
TACTCGG7200.089.1590653
GCTACTC15250.082.630471
TCGGGAG8050.079.744756
ACTCGGG8400.077.837274
GCTGGGA42900.077.589869
GGAGGCT36500.077.189769
CTCGGGA8950.074.382235
AGTAGCT17450.073.5753865
GTAGCTG18150.072.047726
GAGTAGC9900.069.646134
TAGCTGG20350.069.516327
CCCGAGT6850.067.682791
AAAGTGC30600.067.597724
AGTGCTG31950.064.741486
AAGTGCT31650.064.6039355
GTGCTGG33050.063.306087
CCAAAGT33450.062.5490532
Produced by FastQC (version 0.11.5)