FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754032
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA4500.0102.0886842
TACTCGG7050.098.5798953
CTACTCG7050.095.2382052
CGAGTAG4800.083.437873
GCTACTC17400.080.560691
CCCGAGT6000.080.4931
TCGGGAG9000.079.8385856
CTCGGGA9050.078.095895
GCTGGGA41400.076.8225869
ACTCGGG8900.074.1179664
GGAGGCT35150.074.0614859
GAGTAGC11350.066.421644
GTGCTGG31650.065.875677
AGTAGCT18550.064.771175
CCCAAAG38300.064.279581
CCAAAGT35500.063.7086452
AAAGTGC32750.063.3033754
AAGTGCT35200.061.2398155
GTAGCTG18050.060.691976
TGCTGGG36300.060.357588
Produced by FastQC (version 0.11.5)