FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666422
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA47500.098.211619
CTACTCG5250.093.0608752
TACTCGG5650.086.4724963
AGTGCTG36100.081.6392756
AAAGTGC35900.081.216074
GCTACTC15950.081.024791
GTGCTGG35550.080.685697
CCCAAAG40100.080.570161
CCAAAGT37400.079.2229462
AAGTGCT36950.078.908185
TGCTGGG39850.076.329738
TCGGGAG7700.073.684566
GTAGCTG22250.073.666176
CAAAGTG38850.073.020743
TAGCTGG24300.072.640117
GGAGGCT33850.071.701269
ACTCGGG7750.071.175594
GAGTAGC14450.070.894124
AGTAGCT22400.069.654945
CTCGGGA8250.068.7722555
Produced by FastQC (version 0.11.5)