FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666422
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6050.0123.667413
CTACTCG6100.0122.653752
ACTCGGG7500.0101.341854
CTCGGGA7650.099.354755
CGAGTAG3750.098.174923
TCGGGAG8050.095.893156
GCTACTC16150.092.654841
GGAGGCT33050.089.474239
CCGAGTA4400.089.0699842
CCCGAGT5500.079.8930741
GAGTAGC10850.078.8084644
GCTGGGA37850.078.127439
GTAGCTG16700.071.82496
AGTAGCT17450.070.779595
TAGCTGG19000.067.505677
GGGAGGC38100.067.016798
GTGCTGG28050.065.624957
CCAAAGT32400.065.611222
AAGTGCT30300.064.671275
CCCAAAG35300.064.258231
Produced by FastQC (version 0.11.5)