FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725386
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG15500.094.413162
TACTCGG15600.093.807953
CGAGTAG12800.083.403533
CCGAGTA13200.082.69362
GCTACTC37800.079.015451
ACTCGGG19700.076.111144
GCTGGGA114900.075.060829
AGTAGCT46000.071.9706955
CTCGGGA20200.071.851935
TCGGGAG21600.070.526836
GTAGCTG49200.069.727716
GAGTAGC28400.068.4228444
GGAGGCT88600.067.01569
CCCGAGT17000.066.325931
CCCAAAG91950.065.8785551
TAGCTGG56150.064.942397
CCAAAGT86000.063.741492
AAGTGCT83050.061.239365
AAAGTGC79850.061.139794
CAAAGTG88200.058.6155853
Produced by FastQC (version 0.11.5)