FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679353
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG651.4755915E-598.2439961
CTACTCG5750.088.846752
TACTCGG5750.086.070293
GCTACTC14750.083.340881
GCTGGGA46150.079.909739
AGTAGCT21050.077.35845
CGAGTAG5650.076.2912453
CCGAGTA6150.075.280462
ACTCGGG7100.071.9533544
GGAGGCT34250.071.782659
GTAGCTG22350.069.28736
TAGCTGG23300.069.2029957
GAGTAGC12800.067.350874
TCGGGAG8150.064.642146
CGGAGGT7650.060.519597
CCCAAAG41950.058.2262571
GTGCTGG34900.058.0948567
CCAAAGT37100.058.0923962
CCCGAGT7800.057.3090021
AGTGCTG36650.057.0632746
Produced by FastQC (version 0.11.5)