FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764615
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6750.085.8969652
TACTCGG7300.085.865173
GCTGGGA51800.076.839389
CGTTCGG908.1283615E-569.6461942
CCGAGTA6300.069.646192
CGAGTAG5900.069.055973
GTGCTGG39750.068.5949257
AAAGTGC40550.068.400974
AGTGCTG40550.068.014536
CCCAAAG48800.067.755181
CCAAAGT44650.067.3844452
GCTACTC16850.066.959541
TCGGGAG9000.066.163896
GGAGGCT39650.065.606189
AAGTGCT41550.065.246165
CAAAGTG45150.064.902853
TGCTGGG46400.063.492118
CTCGGGA9650.063.331125
ACTCGGG10150.060.2113654
TAGCTGG23900.058.354187
Produced by FastQC (version 0.11.5)