FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764615
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5150.078.411873
CTACTCG5050.077.612682
CGAGTAG5500.066.943623
TCGGGAG8050.064.9182746
CCGAGTA5350.064.38052
GCTGGGA41300.063.8429689
GCTACTC14400.063.5094531
GGAGGCT34800.061.4332359
CTCGGGA7350.061.4053765
CCCGAGT7300.060.198961
ACTCGGG7300.058.571964
TAGCTGG18600.055.5541427
CCAAAGT34500.054.7379042
CCCAAAG38050.054.0009731
AGTGCTG31850.053.3257266
AGTAGCT16350.053.029225
GTGCTGG33050.051.7489057
GAGTAGC11450.050.8277324
CAAAGTG36200.050.526863
GTAGCTG17700.050.3266646
Produced by FastQC (version 0.11.5)