FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434151
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG3950.0118.965752
TACTCGG4350.0111.751423
GCTGGGA31750.0109.727589
TCGGGAG4900.095.900966
GCTACTC11100.090.5085751
AAAGTGC24950.090.274544
GTGCTGG25000.088.797687
CCCAAAG29750.088.236671
GGAGGCT25850.087.758379
AAGTGCT25950.087.420185
CCAAAGT26900.087.344742
AGTGCTG24950.087.027266
CAAAGTG26300.083.792333
TGCTGGG28200.083.3182148
TAGCTGG16750.083.196437
AGTAGCT14850.082.929345
ACTCGGG5500.082.492874
TAGCGGG803.8391587E-581.0197757
GTAGCTG16200.080.019546
CTCGGGA5700.079.598385
Produced by FastQC (version 0.11.5)