FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434151
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG3950.0124.062712
TACTCGG4100.0122.439063
GCTACTC10100.0105.322991
ACTCGGG5100.0100.7749944
CTCGGGA5950.096.422595
CGTTGCG501.6873002E-595.619071
TCGGGAG5950.094.413796
GCTGGGA26450.092.636639
CGAGTAG2600.091.941423
CCGAGTA2850.088.07022
GGAGGCT21600.086.876129
CGGAGGT4800.082.1726467
GTGCTGG20250.076.731367
AGTAGCT11250.076.4952555
GTAGCTG11600.076.247966
AGTGCTG20150.075.925816
AAGTGCT19600.073.7876745
GAGTAGC6750.072.599664
TGCTGGG22050.072.093748
CCAAAGT21450.071.881472
Produced by FastQC (version 0.11.5)