FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093434
Sequences flagged as poor quality0
Sequence length35-351
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT351.8435596E-4130.990924
CTACTCG7700.073.43432
TACTCGG7350.072.7727363
GCTACTC18750.064.389311
GGAGGCT40550.061.0537159
GCTGGGA56200.059.279999
ACTCGGG9850.055.8539924
CCCAAAG50650.055.8187641
CCGCGAT900.0078319650.940912
GTGCTGG43850.050.5343137
TGCTGGG48600.049.368668
TCGGGAG10550.049.250936
AGTGCTG44950.048.6176876
CTCGGGA10750.048.334635
CGGAGGT7650.047.944397
CCAAAGT51450.047.8220832
GGGAGGC47950.046.850768
CCGAGTA5900.046.6238862
AAAGTGC47600.046.2320824
CGGGAGG14850.045.2808077
Produced by FastQC (version 0.11.5)