FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456500
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG3850.0117.629782
CTCGGGA5100.0107.827315
TACTCGG4400.0106.6020053
TCGGGAG5600.098.199876
GCTGGGA36850.097.8785259
AGTAGCT16050.093.7190555
GCTACTC11500.084.386591
CCCAAAG30000.084.10531
GTAGCTG18150.083.766676
ACTCGGG5950.081.5500644
AAAGTGC26200.079.0184864
TAGCTGG19550.078.595357
CCAAAGT29100.078.369332
GAGTAGC10200.077.699094
AAGTGCT27650.077.214495
GGAGGCT28400.076.883919
CGAGTAG3650.075.3314063
GTGCTGG27350.075.1045767
CAAAGTG29000.074.735483
AGTGCTG28450.073.9062356
Produced by FastQC (version 0.11.5)