FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783064
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6600.081.412313
CGAGTAG5350.079.756323
CTACTCG6550.074.79552
CCGAGTA6850.066.905612
CCCGAGT7850.066.435371
GGAGGCT39100.062.6484159
GCTGGGA50900.062.0965279
ACTCGGG8700.059.944564
GAGTAGC13100.057.9061934
CTCGGGA8800.057.467515
GCTACTC17800.055.9339751
TCGGGAG8850.053.571416
AGTAGCT24200.052.8962335
CCCAAAG42150.052.116151
GTAGCTG24850.051.512636
TAGCTGG26800.050.7129337
GTGCTGG38700.048.186597
AGTGCTG39350.046.587396
AAGTGCT39000.046.1950385
CGGAGGT8800.044.8964927
Produced by FastQC (version 0.11.5)