FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6866825
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA53200.056.4947472
CGAGTAG50150.054.802873
GCTGGGA455300.052.66839
CCCGAGT69950.048.710911
CTACTCG51900.046.7614252
TACTCGG53650.045.5356943
GAGTAGC130950.042.3439874
GTGCTGG326700.042.2592857
CCCAAAG386700.042.061471
GTAGCTG224250.041.78446
GGAGGCT328200.041.0867239
TAGCTGG250550.040.5415887
AGTAGCT226850.039.817655
TGCTGGG382550.039.366748
AGTGCTG344150.039.089116
CCAAAGT372950.038.914842
AAAGTGC340750.038.5829624
GCTACTC140100.037.398791
CGGAGGT59500.037.2769367
AAGTGCT359850.036.669065
Produced by FastQC (version 0.11.5)