FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828369
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA51750.043.8380249
CCGAGTA6550.041.1749342
CGAGTAG6000.039.6611483
CCAAAGT40400.035.7343032
GTGCTGG36800.035.3501557
CGCAGTA1850.002670402634.3015331
CCCAAAG47000.034.091711
CCCGAGT8500.033.595331
TGCTGGG43300.032.2418568
AGTGCTG38100.032.062036
GAGTAGC16000.030.7373924
AAGTGCT40000.030.1424735
TAGCTGG30550.030.1191047
AAAGTGC40650.029.660494
CAAAGTG44200.029.43183
AGTAGCT26000.029.2882335
TACTCGG7101.8189894E-1229.04763
GGAGGCT38300.028.9950969
GTAGCTG25750.028.956496
CTACTCG7104.9112714E-1126.813172
Produced by FastQC (version 0.11.5)