FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525078
Sequences flagged as poor quality0
Sequence length35-351
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG250.009985924129.301593
CCGAGTA6100.0113.9337842
CGAGTAG6000.0113.1388853
TACTCGG5600.0103.903063
AGTAGCT21250.0100.398885
GCTGGGA50500.099.8566749
CTACTCG5600.095.244482
GTAGCTG22750.092.3582846
GAGTAGC14200.092.195684
TAGCTGG24750.092.078417
AAGTGCT33050.089.493915
GGAGGCT34600.087.820449
AAAGTGC32500.087.029924
AGTGCTG35100.083.806596
ACTCGGG7150.083.6391454
GCTACTC16050.083.58281
CCCGAGT8350.083.2330551
CCCAAAG38800.082.896321
CCAAAGT36350.082.2585752
GTGCTGG36600.081.2551047
Produced by FastQC (version 0.11.5)