FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525078
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6200.0117.717763
CTACTCG6300.0112.050892
GCTACTC14000.0103.4096761
CCGAGTA4050.091.582082
CGAGTAG3750.089.336843
GCTGGGA41500.088.798679
GGAGGCT33400.085.6160669
TCGGGAG8600.084.866296
ACTCGGG8700.083.8908164
AAAGTGC28000.083.325974
AGTGCTG27600.082.366076
GAGTAGC10050.082.146094
CCAAAGT30800.081.577872
GTGCTGG29400.079.358077
AAGTGCT29100.079.3538745
CCCAAAG32300.079.271131
GTAGCTG17350.078.6156856
AGTAGCT16900.077.8771065
CAAAGTG30900.077.8289953
CTCGGGA9300.074.6189045
Produced by FastQC (version 0.11.5)