FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781408
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT803.5125413E-799.470743
CACGCGA959.770574E-783.764842
CGCGATG1102.3357788E-672.3423544
TACTCGG6700.071.262633
CTACTCG6400.069.629522
CCGAGTA5850.062.5730482
CGAGTAG5750.060.893393
CCCAAAG40500.060.517511
GCTGGGA56000.059.6824469
GAGTAGC14750.053.9502334
ACTCGGG9150.053.9207534
CTCGGGA8800.052.4482085
GCTACTC15850.052.2142941
GTGCTGG39700.051.3138777
GGAGGCT39450.051.2356269
CCAAAGT41550.050.5613022
AGTGCTG40500.050.300276
AAAGTGC39650.050.17444
AGTAGCT24500.050.0195735
TCGGGAG9850.048.473056
Produced by FastQC (version 0.11.5)