FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189340
Sequences flagged as poor quality0
Sequence length35-351
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7300.072.179652
GCTGGGA64400.066.658069
CGAGTAG7650.064.8257143
TACTCGG8700.060.5645373
GGAGGCT49750.059.497549
TGCTGGG57750.055.280758
GTGCTGG53000.055.2642977
AAAGTGC55100.052.595524
GCTACTC22700.052.568261
AGTGCTG56950.052.2475856
CTACTCG9600.051.6579862
AAGTGCT57850.051.4347425
CCCAAAG61700.051.239361
CCAAAGT61900.048.5701872
CCCGAGT11700.047.684291
CAAAGTG60950.047.5473823
GAGTAGC19950.045.8318944
CGGAGGT11250.045.4590267
ACTCGGG12100.044.8271754
TCGGGAG11950.044.093016
Produced by FastQC (version 0.11.5)