FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975277
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2050.034.485581
CGAGTAG10350.026.3639533
CCGAGTA10950.023.6360782
GAGTAGC25200.021.6558324
ACGTCGA2206.134542E-821.4228611
GCTGGGA87350.021.3049099
CTACTCG8850.021.2688262
TACTCGG9400.020.5200733
GTAGCTG45100.020.1115046
AGTAGCT44250.019.9876635
TAGCTGG47200.019.8133777
GCTACTC22000.019.709031
CCCAAAG67650.019.5766621
CCAAAGT63200.019.1356332
CCCGAGT15100.018.7272681
GGAGGCT58550.018.5409729
GTGCTGG61500.017.651617
AAAGTGC58400.017.561484
CAAAGTG66750.017.5497533
AGTGCTG63250.016.9409646
Produced by FastQC (version 0.11.5)